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mouse nerve growth factor 2 5s n 100 alomone labs cat n 240  (Alomone Labs)


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    Alomone Labs mouse nerve growth factor 2 5s n 100 alomone labs cat n 240
    Mouse Nerve Growth Factor 2 5s N 100 Alomone Labs Cat N 240, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nerve+growth+factor+ngf/Native+mouse+NGF+2%2E5S+protein/pm41250640-41-14-20
    Average 93 stars, based on 97 article reviews
    mouse nerve growth factor 2 5s n 100 alomone labs cat n 240 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Neuropathic Pain and Distinct CASPR2 Autoantibody IgG Subclasses Drive Neuronal Hyperexcitability
    Article Snippet: .. The somatic compartment contained from day 1 in culture: 10 ng/ mL nerve growth factor (NGF) (N-100, Alomone Labs, Jerusalem, Israel), 5 ng/mL brain-derived neurotrophic factor (BDNF) and 5 ng/mL ciliary neurotrophic factor (CNTF) (both prepared by M. Sendtner, Institute for Clinical Neurobiology, University of Würzburg), and 5 ng/mL recombinant human glial cell line-derived neurotrophic factor (PeproTech, Cranbury, NJ, US). .. The somatic compartment contained from day 1 in culture: 10 ng/ mL nerve growth factor (NGF) (N-100, Alomone Labs, Jerusalem, Israel), 5 ng/mL brain-derived neurotrophic factor (BDNF) and 5 ng/mL ciliary neurotrophic factor (CNTF) (both prepared by M. Sendtner, Institute for Clinical Neurobiology, University of Würzburg), and 5 ng/mL recombinant human glial cell line-derived neurotrophic factor (PeproTech, Cranbury, NJ, US).

    Article Title: Neuropathic Pain and Distinct CASPR2 Autoantibody IgG Subclasses Drive Neuronal Hyperexcitability
    Article Snippet: .. The somatic compartment contained from day 1 in culture: 10 ng/mL nerve growth factor (NGF) (N-100, Alomone Labs, Jerusalem, Israel), 5 ng/mL brain-derived neurotrophic factor (BDNF) and 5 ng/mL ciliary neurotrophic factor (CNTF) (both prepared by M. Sendtner, Institute for Clinical Neurobiology, University of Würzburg), and 5 ng/mL recombinant human glial cell line-derived neurotrophic factor (PeproTech, Cranbury, NJ, US). ..

    Article Title: Neuropathic pain and distinct CASPR2 autoantibody IgG subclasses drive neuronal hyperexcitability
    Article Snippet: .. The somatic compartment contained from day 1 in culture (DIV): 10ng/ml nerve growth factor (NGF) (N-100, Alomone Labs, Jerusalem, Israel), 5ng/ml brain-derived neurotrophic factor (BDNF) (prepared by M. Sendtner, Institute of Clinical Neurobiology, University of Würzburg), 5ng/ml ciliary neurotrophic factor (CNTF) (prepared by M. Sendtner, Institute of Clinical Neurobiology, University of Würzburg) and 5ng/ml recombinant human glial cell line-derived neurotrophic factor (GDNF) (PeproTech, Cranbury, New Jersey, USA). ..

    Incubation:

    Article Title: Tight Spaces, Tighter Signals: Spatial Constraints as Drivers of Peripheral Myelination
    Article Snippet: DRG were dissected from Sprague Dawley rat embryos at embryonic day 14.5 (E14.5) or from C57/B6 E13.5 mouse embryos as previously described [ ]. .. The dissected DRG were enzymatically dissociated by incubation in 0.25% trypsin (GIBCO, Waltham, MA, USA) at 37 °C for 1 h. Cells were collected via gentle centrifugation (900 rpm for 15 min), resuspended in C-medium (DMEM supplemented with 10% FCS, 1% penicillin-streptomycin (P/S), 200 mM L-glutamine, and 50 μg/mL 2.5S nerve growth factor NGF; Alomone Labs, Jerusalem, Israel), and plated overnight at 37 °C with 5% CO 2 at a density of 1.5 DRG per 12 mm glass coverslip (VWR International LLC, Radnor, PA, USA) pre-coated with 50 μg/mL ammoniated rat collagen (Cultrex 3440-100-01; R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Tight Spaces, Tighter Signals: Spatial Constraints as Drivers of Peripheral Myelination.
    Article Snippet: DRG were dissected from Sprague Dawley rat embryos at embryonic day 14.5 (E14.5) or from C57/B6 E13.5 mouse embryos as previously described [32]. .. The dissected DRG were enzymatically dissociated by incubation in 0.25% trypsin (GIBCO, Waltham, MA, USA) at 37 ◦C for 1 h. Cells were collected via gentle centrifugation (900 rpm for 15 min), resuspended in C-medium (DMEM supplemented with 10% FCS, 1% penicillin-streptomycin (P/S), 200 mM L-glutamine, and 50 μg/mL 2.5S nerve growth factor NGF; Alomone Labs, Jerusalem, Israel), and plated overnight at 37 ◦C with 5% CO2 at a density of 1.5 DRG per 12 mm glass coverslip (VWR International LLC, Radnor, PA, USA) pre-coated with 50 μg/mL ammoniated rat collagen (Cultrex 3440-100-01; R&D Systems, Minneapolis, MN, USA). ..

    Gentle:

    Article Title: Tight Spaces, Tighter Signals: Spatial Constraints as Drivers of Peripheral Myelination
    Article Snippet: DRG were dissected from Sprague Dawley rat embryos at embryonic day 14.5 (E14.5) or from C57/B6 E13.5 mouse embryos as previously described [ ]. .. The dissected DRG were enzymatically dissociated by incubation in 0.25% trypsin (GIBCO, Waltham, MA, USA) at 37 °C for 1 h. Cells were collected via gentle centrifugation (900 rpm for 15 min), resuspended in C-medium (DMEM supplemented with 10% FCS, 1% penicillin-streptomycin (P/S), 200 mM L-glutamine, and 50 μg/mL 2.5S nerve growth factor NGF; Alomone Labs, Jerusalem, Israel), and plated overnight at 37 °C with 5% CO 2 at a density of 1.5 DRG per 12 mm glass coverslip (VWR International LLC, Radnor, PA, USA) pre-coated with 50 μg/mL ammoniated rat collagen (Cultrex 3440-100-01; R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Tight Spaces, Tighter Signals: Spatial Constraints as Drivers of Peripheral Myelination.
    Article Snippet: DRG were dissected from Sprague Dawley rat embryos at embryonic day 14.5 (E14.5) or from C57/B6 E13.5 mouse embryos as previously described [32]. .. The dissected DRG were enzymatically dissociated by incubation in 0.25% trypsin (GIBCO, Waltham, MA, USA) at 37 ◦C for 1 h. Cells were collected via gentle centrifugation (900 rpm for 15 min), resuspended in C-medium (DMEM supplemented with 10% FCS, 1% penicillin-streptomycin (P/S), 200 mM L-glutamine, and 50 μg/mL 2.5S nerve growth factor NGF; Alomone Labs, Jerusalem, Israel), and plated overnight at 37 ◦C with 5% CO2 at a density of 1.5 DRG per 12 mm glass coverslip (VWR International LLC, Radnor, PA, USA) pre-coated with 50 μg/mL ammoniated rat collagen (Cultrex 3440-100-01; R&D Systems, Minneapolis, MN, USA). ..

    Centrifugation:

    Article Title: Tight Spaces, Tighter Signals: Spatial Constraints as Drivers of Peripheral Myelination
    Article Snippet: DRG were dissected from Sprague Dawley rat embryos at embryonic day 14.5 (E14.5) or from C57/B6 E13.5 mouse embryos as previously described [ ]. .. The dissected DRG were enzymatically dissociated by incubation in 0.25% trypsin (GIBCO, Waltham, MA, USA) at 37 °C for 1 h. Cells were collected via gentle centrifugation (900 rpm for 15 min), resuspended in C-medium (DMEM supplemented with 10% FCS, 1% penicillin-streptomycin (P/S), 200 mM L-glutamine, and 50 μg/mL 2.5S nerve growth factor NGF; Alomone Labs, Jerusalem, Israel), and plated overnight at 37 °C with 5% CO 2 at a density of 1.5 DRG per 12 mm glass coverslip (VWR International LLC, Radnor, PA, USA) pre-coated with 50 μg/mL ammoniated rat collagen (Cultrex 3440-100-01; R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Tight Spaces, Tighter Signals: Spatial Constraints as Drivers of Peripheral Myelination.
    Article Snippet: DRG were dissected from Sprague Dawley rat embryos at embryonic day 14.5 (E14.5) or from C57/B6 E13.5 mouse embryos as previously described [32]. .. The dissected DRG were enzymatically dissociated by incubation in 0.25% trypsin (GIBCO, Waltham, MA, USA) at 37 ◦C for 1 h. Cells were collected via gentle centrifugation (900 rpm for 15 min), resuspended in C-medium (DMEM supplemented with 10% FCS, 1% penicillin-streptomycin (P/S), 200 mM L-glutamine, and 50 μg/mL 2.5S nerve growth factor NGF; Alomone Labs, Jerusalem, Israel), and plated overnight at 37 ◦C with 5% CO2 at a density of 1.5 DRG per 12 mm glass coverslip (VWR International LLC, Radnor, PA, USA) pre-coated with 50 μg/mL ammoniated rat collagen (Cultrex 3440-100-01; R&D Systems, Minneapolis, MN, USA). ..

    Glass:

    Article Title: Tight Spaces, Tighter Signals: Spatial Constraints as Drivers of Peripheral Myelination
    Article Snippet: DRG were dissected from Sprague Dawley rat embryos at embryonic day 14.5 (E14.5) or from C57/B6 E13.5 mouse embryos as previously described [ ]. .. The dissected DRG were enzymatically dissociated by incubation in 0.25% trypsin (GIBCO, Waltham, MA, USA) at 37 °C for 1 h. Cells were collected via gentle centrifugation (900 rpm for 15 min), resuspended in C-medium (DMEM supplemented with 10% FCS, 1% penicillin-streptomycin (P/S), 200 mM L-glutamine, and 50 μg/mL 2.5S nerve growth factor NGF; Alomone Labs, Jerusalem, Israel), and plated overnight at 37 °C with 5% CO 2 at a density of 1.5 DRG per 12 mm glass coverslip (VWR International LLC, Radnor, PA, USA) pre-coated with 50 μg/mL ammoniated rat collagen (Cultrex 3440-100-01; R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Tight Spaces, Tighter Signals: Spatial Constraints as Drivers of Peripheral Myelination.
    Article Snippet: DRG were dissected from Sprague Dawley rat embryos at embryonic day 14.5 (E14.5) or from C57/B6 E13.5 mouse embryos as previously described [32]. .. The dissected DRG were enzymatically dissociated by incubation in 0.25% trypsin (GIBCO, Waltham, MA, USA) at 37 ◦C for 1 h. Cells were collected via gentle centrifugation (900 rpm for 15 min), resuspended in C-medium (DMEM supplemented with 10% FCS, 1% penicillin-streptomycin (P/S), 200 mM L-glutamine, and 50 μg/mL 2.5S nerve growth factor NGF; Alomone Labs, Jerusalem, Israel), and plated overnight at 37 ◦C with 5% CO2 at a density of 1.5 DRG per 12 mm glass coverslip (VWR International LLC, Radnor, PA, USA) pre-coated with 50 μg/mL ammoniated rat collagen (Cultrex 3440-100-01; R&D Systems, Minneapolis, MN, USA). ..



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    Image Search Results


    MSM-DTM adsorbs inflammatory cytokines, suppresses TSPCs inflammation and senescence, and promotes tenogenic differentiation and migration (A) The remaining concentrations of TNF-α, IL-1β, IL-6, IFN-γ, LPS, and NGF were detected by ELISA after co-culture with different concentrations of MSM. (B and C) RT-qPCR of IL-6 and CCL-2 in TSPCs under different treatments. (D) Western blot of COL1A2, MMP3, and TNMD. (E) ELISA of TNF-α, IL-6, and IFN-γ in supernatants. (F)Western blot of p16 and p21 after 7 days of treatment. (G) β-Galactosidase staining analysis after 7 days of treatment. Scale bars, 100 μm. (H and I) Immunofluorescence and quantification of TNMD and TNC after 14-day treatment. Scale bars, 50 μm. (J) Relative mRNA expression of tenogenic differentiation markers SCX and TNC. (K and L) Representative images and quantification of Transwell assay. Scale bars, 100 μm. (M and N) Representative images and quantification of wound healing assay. Scale bars, 100 μm. Statistical comparisons were performed with one-way ANOVA with Tukey’s multiple comparisons test. Data are presented as the mean ± SD, n = 3. Ns, no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 between groups.

    Journal: Cell Reports Medicine

    Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

    doi: 10.1016/j.xcrm.2026.102937

    Figure Lengend Snippet: MSM-DTM adsorbs inflammatory cytokines, suppresses TSPCs inflammation and senescence, and promotes tenogenic differentiation and migration (A) The remaining concentrations of TNF-α, IL-1β, IL-6, IFN-γ, LPS, and NGF were detected by ELISA after co-culture with different concentrations of MSM. (B and C) RT-qPCR of IL-6 and CCL-2 in TSPCs under different treatments. (D) Western blot of COL1A2, MMP3, and TNMD. (E) ELISA of TNF-α, IL-6, and IFN-γ in supernatants. (F)Western blot of p16 and p21 after 7 days of treatment. (G) β-Galactosidase staining analysis after 7 days of treatment. Scale bars, 100 μm. (H and I) Immunofluorescence and quantification of TNMD and TNC after 14-day treatment. Scale bars, 50 μm. (J) Relative mRNA expression of tenogenic differentiation markers SCX and TNC. (K and L) Representative images and quantification of Transwell assay. Scale bars, 100 μm. (M and N) Representative images and quantification of wound healing assay. Scale bars, 100 μm. Statistical comparisons were performed with one-way ANOVA with Tukey’s multiple comparisons test. Data are presented as the mean ± SD, n = 3. Ns, no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 between groups.

    Article Snippet: The remaining cytokines in the supernatant were quantified using ELISA kits for TNF-α (ABclonal, China), IL-1β (ABclonal, China), IL-6 (ABclonal, China), IFN-γ (ABclonal, China), and NGF (ABclonal, China), and LPS (ABclonal, China), following the manufacturers’ protocols.

    Techniques: Migration, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Quantitative RT-PCR, Western Blot, Staining, Immunofluorescence, Expressing, Transwell Assay, Wound Healing Assay

    Journal: Cell Reports Medicine

    Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

    doi: 10.1016/j.xcrm.2026.102937

    Figure Lengend Snippet:

    Article Snippet: The remaining cytokines in the supernatant were quantified using ELISA kits for TNF-α (ABclonal, China), IL-1β (ABclonal, China), IL-6 (ABclonal, China), IFN-γ (ABclonal, China), and NGF (ABclonal, China), and LPS (ABclonal, China), following the manufacturers’ protocols.

    Techniques: Recombinant, Lysis, Hydroxyproline Assay, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Staining, Software

    hUC‐MSCs exert therapeutic effects in the PND mouse model via β‐NGF. (A) 20‐Human Neuro Discovery array analysis between blank‐medium and MSC‐conditioned medium (MSC‐CM) (B) ELISA quantification of β‐NGF levels in blank‐medium and MSC‐CM ( n = 3). (C, D) Representative Western blot bands and quantitative analysis of β‐NGF protein expression in hippocampal tissues from CON, CON‐MSC, SUR, and SUR‐MSC groups. (E) Total distance traveled by mice in OFT ( n = 9). (F) Alternative corrections (%) in Y‐maze test ( n = 9). (G) Novel object preference (%) in NOR ( n = 9). (H) Representative GFAP, NeuN and IBA1 immunostaining images of hippocampal CA1 region. (I) Representative Golgi staining images (J) Quantitative analysis of NeuN positive area percentage in hippocampal CA1 region ( n = 6). (K) Quantitative analysis of GFAP positive area percentage in hippocampal CA1 region ( n = 6). (L) Quantitative analysis of IBA1 positive area percentage in hippocampal CA1 region ( n = 6). (M) Quantitative analysis of dendritic spine density in hippocampal CA1 region. Representative Western blot bands (N) and quantitative analysis of β‐NGF (O), p‐TrkA/TrkA (P), STMN2 (Q) and NMNAT2 (R) protein expression in hippocampal tissues from CON, SUR, SUR‐MSC NC and SUR‐MSC NGFsiRNA groups ( n = 6). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: hUC ‐ MSCs via β‐ NGF Alleviate Cognitive Impairment After Tibial Fracture Surgery by Regulating the STMN2 / NMNAT2 ‐ SARM1 ‐ NF ‐ κB Signaling Pathway

    doi: 10.1002/cns.71062

    Figure Lengend Snippet: hUC‐MSCs exert therapeutic effects in the PND mouse model via β‐NGF. (A) 20‐Human Neuro Discovery array analysis between blank‐medium and MSC‐conditioned medium (MSC‐CM) (B) ELISA quantification of β‐NGF levels in blank‐medium and MSC‐CM ( n = 3). (C, D) Representative Western blot bands and quantitative analysis of β‐NGF protein expression in hippocampal tissues from CON, CON‐MSC, SUR, and SUR‐MSC groups. (E) Total distance traveled by mice in OFT ( n = 9). (F) Alternative corrections (%) in Y‐maze test ( n = 9). (G) Novel object preference (%) in NOR ( n = 9). (H) Representative GFAP, NeuN and IBA1 immunostaining images of hippocampal CA1 region. (I) Representative Golgi staining images (J) Quantitative analysis of NeuN positive area percentage in hippocampal CA1 region ( n = 6). (K) Quantitative analysis of GFAP positive area percentage in hippocampal CA1 region ( n = 6). (L) Quantitative analysis of IBA1 positive area percentage in hippocampal CA1 region ( n = 6). (M) Quantitative analysis of dendritic spine density in hippocampal CA1 region. Representative Western blot bands (N) and quantitative analysis of β‐NGF (O), p‐TrkA/TrkA (P), STMN2 (Q) and NMNAT2 (R) protein expression in hippocampal tissues from CON, SUR, SUR‐MSC NC and SUR‐MSC NGFsiRNA groups ( n = 6). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: The supernatant was harvested following the manufacturer's instructions, and the concentrations of IL‐1β (HUABIO, China, EM0001), IL‐6 (LIANKE, China, EK206), TNF‐α (HUABIO, China, EM0010), mouse (HUABIO, China, RK00154), and human β‐NGF (Abclonal, China, RK03064) were quantified using ELISA kits.

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Immunostaining, Staining